Open AccessQuantitative immunophenotypic analysis of antigen‐presenting cells involved in ectromelia virus antigen presentation in BALB /c and C 57 BL /6 mice
Author(s) -
SzulcDąbrowska Lidia,
Gieryńska Małgorzata,
BoratyńskaJasińska Anna,
Martyniszyn Lech,
Winnicka Anna,
Niemiałtowski Marek G.
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12054
Subject(s) - ectromelia virus , antigen presentation , biology , cd11c , antigen , antigen presenting cell , immunology , immune system , t cell , population , dendritic cell , myeloid , virology , phenotype , medicine , biochemistry , environmental health , gene , vaccinia , recombinant dna
During mousepox in resistant ( C 57BL/6) or susceptible ( BALB /c) strains of mice, stimulation of T h1 or T h2 cytokine immune response, respectively, is observed. Because mechanisms of different polarization of T cells remain elusive, in this study, we quantitatively assessed the phenotype of antigen‐presenting cells ( APC s) involved in ectromelia virus ( ECTV ) antigen presentation and cluster formation with effector cells in secondary lymphoid organs of BALB /c and C 57 BL /6 mice. We showed that both strains of mice display similar dynamics and kinetics of viral antigen presentation by CD 11c + , CD 11b + , and CD 19 + cells. CD 11c + and CD 11b + cells highly participated in viral antigen presentation during all stages of mousepox, whereas CD 19 + cells presented viral peptides later in infection. The main population of dendritic cells ( DC s) engaged in ECTV antigen presentation and cell junction formation with effector cells was a population of myeloid CD 11b + DC s (m DC s). We suggest that, on the one hand, ECTV may differentially affect the functions of APC s depending on the strain of mice. On the other hand, we suggest that some types of APC s, such as m DC s or other DC s subsets, have different abilities to direct the shape of immune response depending on the host resistance to mousepox.