Open AccessResveratrol role in S taphylococcus aureus ‐induced corneal inflammation
Author(s) -
Marino Andreana,
Santoro Giuseppe,
Spataro Francesca,
Lauriano Eugenia R.,
Pergolizzi Simona,
Cimino Francesco,
Speciale Antonio,
Nostro Antonia,
Bisignano Giuseppe,
Dugo Giacomo
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12046
Subject(s) - staphylococcus aureus , resveratrol , in vivo , inflammation , cornea , ex vivo , microbiology and biotechnology , keratitis , tlr2 , chemistry , biology , medicine , pharmacology , immunology , bacteria , ophthalmology , genetics , tlr4
The aim of this study was to evaluate the role of trans ‐resveratrol on S taphylococcus aureus ‐induced keratitis. Rabbit corneas (intact corneas, abraded corneas and abraded corneas exposed to inactivated S . aureus strains) were placed in an ex vivo culture model. The abraded corneas exposed to S . aureus were divided into two 1‐h‐treatment sub‐groups: corneas treated with trans ‐resveratrol and corneas treated with vehicle. The tissues were examined by immunohistochemical analyses and quantitative real‐time RT ‐ PCR to determine whether resveratrol could reduce TLR 2‐mediated recognition of S . aureus on epithelial cells and, if so, whether this reduction repressed the expression of inflammatory cytokines. The results demonstrated that resveratrol treatment effectively downregulated cell surface TLR 2 on cells stimulated by S . aureus and reduced the expression of interleukin‐8 gene. In addition, the corneal culture model tested, which is simple and reproducible, could be an alternative to in vivo animal testing for the development of novel specific therapies.