Open AccessSerum antibody response to group II chaperonin from M ethanobrevibacter oralis and human chaperonin CCT
Author(s) -
Hirai Kimito,
Maeda Hiroshi,
Omori Kazuhiro,
Yamamoto Tadashi,
Kokeguchi Susumu,
Takashiba Shogo
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12041
Subject(s) - chaperonin , autoantibody , periodontal pathogen , biology , hsp60 , immunology , immune system , aggressive periodontitis , antibody , periodontitis , medicine , heat shock protein , porphyromonas gingivalis , genetics , gene , hsp70
Both group I ( HSP 60) and group II ( CCT ) chaperonins are targets of autoantibodies. Autoimmune reactions to HSP 60 have been well characterized, while immune reactions to group II chaperonin have not been clarified. Methanobrevibacter oralis is a suspected periodontal pathogen with group II chaperonin. In this study, serum responses to M. oralis chaperonin, human HSP 60, and CCT subunits were examined using sera from patients with periodontitis and autoimmune diseases. In comparison with healthy controls, periodontitis patients showed significantly higher responses to CCT 4 and CCT 8 on dot blot analysis. Signals for CCT 3 and CCT 8 in autoimmune disease patients were significantly higher than in controls. Significant differences were also demonstrated by Western blotting in anti‐ CCT 4 response in both patient groups. All subjects showed strong reactivity to M. oralis chaperonin and faint signals to human HSP 60. Autoantibodies were raised against CCT rather than HSP 60; and CCT 3, CCT 4, and CCT 8 were shown to be the main targets. Host immune systems may be frequently exposed to chaperonins of Archaea in various habitats. Although further studies of the cross‐reactivity between M. oralis chaperonin and human CCT are required, anti‐ CCT autoantibodies may be involved in the pathogenesis of periodontitis and autoimmune diseases.