Open AccessGreen tea epigallocatechin‐3‐gallate attenuates P orphyromonas gingivalis ‐induced atherosclerosis
Author(s) -
Cai Yu,
KuritaOchiai Tomoko,
Hashizume Tomomi,
Yamamoto Masafumi
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12001
Subject(s) - porphyromonas gingivalis , epigallocatechin gallate , chemistry , atheroma , ccl2 , inflammation , apolipoprotein b , oil red o , oxidative stress , tlr4 , endocrinology , medicine , cholesterol , periodontitis , chemokine , biochemistry , antioxidant , in vitro , polyphenol , adipogenesis
The purpose of this study was to determine whether epigallocatechin‐3‐gallate ( EGCG ) ameliorates P orphyromonas gingivalis ‐induced atherosclerosis. EGCG is a polyphenol extract from green tea with health benefits and P . gingivalis is shown here to accelerate atheroma formation in a murine model. Apolipoprotein E knockout mice were administered EGCG or vehicle in drinking water; they were then fed high‐fat diets and injected with P . gingivalis three times a week for 3 weeks. Mice were then killed at 15 weeks. Atherosclerotic plaques in the proximal aorta were determined by Oil Red O staining. Atherosclerosis risk factors in serum, liver or aorta were analysed using cytokine antibody arrays, enzyme‐linked immunosorbent assay and real‐time PCR . Atherosclerotic lesion areas of the aortic sinus caused by P . gingivalis infection decreased in EGCG ‐treated groups, wherein EGCG reduced the production of C‐reactive protein, monocyte chemoattractant protein‐1, and oxidized low‐density lipoprotein ( LDL ), and slightly lowered LDL /very LDL cholesterol in P . gingivalis ‐challenged mice serum. Furthermore, the increase in CCL 2, MMP ‐9, ICAM ‐1, HSP 60, CD 44, LOX ‐1, NOX ‐4, p22phox and i NOS gene expression levels in the aorta of P . gingivalis ‐challenged mice were reduced in EGCG ‐treated mice. However, HO ‐1 m RNA levels were elevated by EGCG treatment, suggesting that EGCG , as a natural substance, inhibits P . gingivalis ‐induced atherosclerosis through anti‐inflammatory and antioxidative effects.