Open AccessS ri L ankan cassava mosaic virus replication associated protein (Rep) triggers transposition of IS 426 in A grobacterium
Author(s) -
Resmi Thulasi R.,
Nivedhitha Sivarajan,
Karthikeyan Chockalingam,
Veluthambi Karuppannan
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12584
Subject(s) - transposition (logic) , agrobacterium , biology , replication (statistics) , virology , mosaic , plant virus , mosaic virus , genetics , virus , transformation (genetics) , gene , computer science , geography , archaeology , artificial intelligence
We report a high rate of IS 426 transposition in Agrobacterium tumefaciens in the presence of the Sri Lankan cassava mosaic virus (SLCMV) replication associated protein gene ( Rep ). Upon conjugal transfer of the binary plasmid pCam‐SLCMV‐ Rep with the SLCMV Rep gene in the sense orientation under the transcriptional control of the Cauliflower mosaic virus (CaMV) 35S promoter into the A. tumefaciens vir helper strain EHA105, the binary plasmid size increased in all 15 transconjugants studied. Southern blot analysis of the transconjugants with the binary plasmid probe revealed that the 35S promoter and its proximal sequences in the T‐DNA were rearranged. The rearranged sequences harboured the 1.3‐kb IS 426 element of A. tumefaciens . Conjugal mobilisation of the binary plasmid pCam‐SLCMV‐as Rep , with the SLCMV Rep gene in antisense orientation, did not cause DNA rearrangement in EHA105. A mutated SLCMV Rep , in which a frame shift mutation caused retention of only 27 of the 351 amino acids, did not cause IS 426 transposition in A. tumefaciens . These findings show that the multifunctional begomoviral Rep protein of SLCMV triggers transposition of IS 426 in Agrobacterium .