Open AccessComplete genome sequence of the bacteriophage YMC /09/04/ R 1988 MRSA BP : a lytic phage from a methicillin‐resistant S taphylococcus aureus isolate
Author(s) -
Jeon Jongsoo,
D'Souza Roshan,
Hong Sung Kuk,
Lee Yangsoon,
Yong Dongeun,
Choi Jongrak,
Lee Kyungwon,
Chong Yunsop
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12580
Subject(s) - lytic cycle , orfs , bacteriophage , staphylococcus aureus , microbiology and biotechnology , genome , biology , virulence , lysogenic cycle , myoviridae , whole genome sequencing , virology , staphylococcal infections , sccmec , methicillin resistant staphylococcus aureus , virus , gene , genetics , bacteria , open reading frame , peptide sequence , escherichia coli
Methicillin‐resistant S taphylococcus aureus ( MRSA ) is an increasing cause of serious infection, both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection have been narrowed due to the limited number of newly developed antimicrobials. Herein, we analyze the completely sequenced genome of a novel virulent phage YMC /09/04/ R 1988 MRSA BP as a potential alternative anti‐ MRSA agent, which lysed clinical isolates from a patient admitted to the hospital due to hip disarticulation. The phage contains a linear double‐stranded DNA genome of 44 459 bp in length, with 33.37% GC content, 62 predicted open reading frames ( ORF s), and annotated functions of only 23 ORF s that are associated with structural assembly, host lysis, DNA replication, and modification. It showed a broad host range (17 of 30 strains) against MRSA strains in clinical isolates.