Open AccessQuantitative volatile compound profiles in fungal cultures of three different Fusarium graminearum chemotypes
Author(s) -
Buśko Maciej,
Kulik Tomasz,
Ostrowska Anna,
Góral Tomasz,
Perkowski Juliusz
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12569
Subject(s) - chemotype , trichothecene , nonanal , fusarium , terpene , hexanal , chemistry , benzene , biology , chromatography , microbiology and biotechnology , botany , organic chemistry , essential oil
Biosynthesis in fungal cultures of 27 Fusarium graminearum isolates of three different chemotypes (3Ac DON , 15Ac DON and NIV ) grown on yeast extract sucrose agar medium was examined in this study. Volatile organic compound ( VOC ) analysis performed by headspace solid phase microextraction GC ‐ MS allowed for determination of various concentrations of six alcohols, 14 aldehydes and ketones, 10 benzene derivatives, one furane, five hydrocarbons and three terpenes. In general, the determined VOC profile in fungal cultures was dominated by hexanal (up to 74%), followed by nonanal (18%) and 2‐methylbutanal (18%). Principal component analysis and discriminant analysis based on VOC s allowed for unambiguous discrimination of all studied isolates into three different groups in accordance with their trichothecene production (chemotypes). Significant differences were revealed between the levels of aldehydes and ketones, benzene derivatives and hydrocarbons in fungal cultures of three F. graminearum chemotypes.