Open AccessA new species of Scytalidium causing slippery scar on cultivated Auricularia polytricha in China
Author(s) -
Peng Weihong,
He Xiaolan,
Wang Yong,
Zhang Yong,
Ye Xiaojin,
Jia Dinghong,
Guo Yong,
Gan Bingcheng,
Zheng Chao,
Yang Zhirong,
Sun Qun
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12564
Subject(s) - internal transcribed spacer , biology , conidium , pathogen , auricularia , mycology , phylogenetic tree , botany , microbiology and biotechnology , genetics , gene
Slippery scar is one of the most destructive diseases encountered in the cultivation of Auricularia polytricha (hairy wood ear); however, the identity of the pathogenic agent has remained uncertain. This study was designed to identify the causative pathogen of slippery scar in A. polytricha and to investigate the taxonomic classification of the pathogen by morphological observations, in vivo pathogenicity tests, and molecular evidences of ITS and RPB 2 sequences. The results showed that the pathogen was a new Scytalidium species, here named Scytalidium auriculariicola . Scytalidium auriculariicola was characterized by its rapid growth rate, the catenate conidia of variable size, and the pale brown to brown chlamydoconidia. Phylogenetic analyses based on internal transcribed spacer regions and RPB 2 sequences on the pathogen isolated and related species supported that S. auriculariicola was a true Scytalidium species. It was congeneric with and close to Scytalidium lignicola , the type species of Scytalidium . However, it differed from the latter species in the size of conidia, 33 different nucleotide bases in ITS sequences and 30 different nucleotide bases in RPB 2 sequences.