Open AccessImpact of rli87 gene deletion on response of Listeria monocytogenes to environmental stress
Author(s) -
Kun Xie,
Qingling Meng,
Qiao Jun,
Yelong Peng,
Tianli Liu,
Cheng Chen,
Yu Ma,
Zhengxiang Hu,
Xuepeng Cai,
Chuangfu Chen
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12561
Subject(s) - listeria monocytogenes , strain (injury) , gene , gene expression , oxidative stress , microbiology and biotechnology , osmotic pressure , osmotic shock , biology , chemistry , biochemistry , bacteria , food science , genetics , anatomy
Listeria monocytogenes (LM) is a zoonotic pathogen that widely adapts to various environments. Recent studies have found that noncoding RNAs (ncRNAs) play regulatory roles in LM responses to environmental stress. To understand the role of ncRNA rli87 in the response regulation, a rli87 deletion strain LM‐Δ rli87 was constructed by homologous recombination and tested for stress responses to high temperature, low temperature, high osmotic pressure, alcohol, acidity, alkaline and oxidative environments, along with LM EGD‐e strain (control). The results showed that compared with LM EGD‐e, LM‐Δ rli87 grew faster ( P < 0.05) at low temperature (30 °C), high temperature (42 °C), and in alkaline condition ( pH = 9), similarly ( P > 0.05) in acidic and high osmatic pressure (10% NaCl) conditions. When cultured in medium containing 3.8% ethanol, the growth was not significantly different between the two strains ( P > 0.05). When cultured at pH 9, they had similar growth rates in the first 5 h ( P > 0.05), but the rates were significantly different after 6 h ( P < 0.05). The expression of rsbV , rsbW , hpt , clpP , and ctsR was upregulated in LM‐∆rli87 compared with LM EGD‐e at pH 9, indicating that the rli87 gene regulated the expression of the five genes in alkaline environment. Our results suggest that the rli87 gene has an important regulatory role in LM's response to temperature (30 and 42 °C), alkaline stresses.