Open AccessThe spore surface of intestinal isolates of Bacillus subtilis
Author(s) -
Sirec Teja,
Cangiano Giuseppina,
Baccigalupi Loredana,
Ricca Ezio,
Isticato Rachele
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12538
Subject(s) - bacillus subtilis , heterologous , microbiology and biotechnology , biology , spore , recombinant dna , biofilm , model organism , strain (injury) , organism , bacillus (shape) , bacteria , genetics , gene , anatomy
Bacillus subtilis has been used for over 50 years as a model organism for biochemistry, genetic, molecular biology and cell biology studies. More recently, its spore has been proposed as a platform to display heterologous proteins and as a vehicle for mucosal vaccination. We characterize here the spore surface of four human intestinal strains of B. subtilis, previously identified as able to grow anaerobically and form biofilm. These properties, lost in laboratory strains, are relevant for the colonization of human mucosal sites and likely to improve the efficiency of strains to be used for mucosal delivery. Our characterization is an essential preliminary step for the development of these intestinal strains as display systems and has indicated that spores of at least one of them are more efficient than the laboratory strain for the non‐recombinant display of two model heterologous proteins.