Open AccessA new high phenyl lactic acid‐yielding L actobacillus plantarum IMAU 10124 and a comparative analysis of lactate dehydrogenase gene
Author(s) -
Zhang Xiqing,
Zhang Shuli,
Shi Yan,
Shen Fadi,
Wang Haikuan
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12483
Subject(s) - lactobacillus plantarum , lactic acid , lactate dehydrogenase , lactobacillus , bacteria , food science , pyruvic acid , chemistry , fermentation , biochemistry , substrate (aquarium) , microbiology and biotechnology , biology , enzyme , ecology , genetics
Phenyl lactic acid ( PLA ) has been widely reported as a new natural antimicrobial compound. In this study, 120 Lactobacillus plantarum strains were demonstrated to produce PLA using high‐performance liquid chromatography. Lactobacillus plantarum IMAU 10124 was screened with a PLA yield of 0.229 g L −1 . Compared with all previous reports, this is the highest PLA ‐producing lactic acid bacteria ( LAB ) when grown in MRS broth without any optimizing conditions. When 3.0 g L −1 phenyl pyruvic acid ( PPA ) was added to the medium as substrate, PLA production reached 2.90 g L −1 , with the highest 96.05% conversion rate. A lowest PLA ‐yielding L. plantarum IMAU 40105 (0.043 g L −1 ) was also screened. It was shown that the conversion from PPA to PLA by lactic dehydrogenase ( LDH ) is the key factor in the improvement of PLA production by LAB . Comparing the LDH gene of two strains, four amino acid mutation sites were found in this study in the LDH of L. plantarum IMAU 10124.