Open AccessIdentification of novel s RNA s in B rucella abortus 2308
Author(s) -
Dong Hao,
Peng Xiaowei,
Wang Ning,
Wu Qingmin
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12433
Subject(s) - transfer rna , biology , gene , brucella , brucella abortus , computational biology , identification (biology) , genome , genetics , rna , virology , brucellosis , ecology
Involved in diverse biological processes, bacterial s RNA s are novel regulators of gene expression involved in a wide array of biological processes. To identify s RNA s in Brucella abortus , we performed a genome‐wide computational prediction with integrated sipht and napp results. In total, 129 s RNA candidates were identified, of which 112 were novel s RNA . Twenty novel s RNA candidates were tested by RT ‐ PCR and seven could be verified. The putative targets of these s RNA s were also predicted and verified. This study provides a significant resource for the future study of s RNA s, as well as how s RNA s influence B. abortus physiology and pathogenesis.