Open AccessInteractions of three soil bacteria species with phyllosilicate surfaces in hybrid silica gels
Author(s) -
Oulkadi Djihad,
BallandBolouBi Clarisse,
Billard Patrick,
Kitzinger Geraldine,
Parrello Damien,
Mustin Christian,
Ba Sylvie
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12421
Subject(s) - chemistry , environmental chemistry , oxalate , shewanella putrefaciens , montmorillonite , organic matter , soil water , microorganism , siderophore , bacteria , botany , nuclear chemistry , inorganic chemistry , biochemistry , organic chemistry , biology , ecology , genetics , gene
To simulate iron consumption in soils, iron leaching from silicate minerals due to three heterotrophic bacterial strains and a chemical treatment was studied using hybrid silica gel ( HSG ) doped with two phyllosilicates, nontronite ( NA u‐2) or low‐iron‐content montmorillonite ( SW y‐2). HSG methodology, a novel way of separating bacteria cells from a colloidal mineral source, consisted in embedding colloidal mineral particles into an amorphous porous silica matrix using a classical sol‐gel procedure. P antoae agglomerans PA 1 and R ahnella aquatilis RA 1 were isolated from silicate‐rich soils, that is, beech and wheat rhizospheres ( V osges, F rance); B urkholderia sp. G 5 was selected from acidic and nutrient‐poor podzol soils ( V osges, F rance). Fe release from clay minerals and production of bacterial metabolites, that is, low molecular weight organic acids ( LMWOA ) and siderophores, were monitored. Two LMWOA profiles were observed with major gluconate production (> 9000 μM) for B urkholderia sp. G 5 and moderate production of lactate, acetate, propionate, formate, oxalate, citrate, and succinate (< 300 μM) for R . aquatilis RA 1 and P . agglomerans PA 1 . HSG demonstrated its usefulness in revealing clay mineral–microorganisms interactions. The effect of bacterial exsudates was clearly separated from physical contact effect.