Open AccessThe pqqC gene is essential for antifungal activity of Pseudomonas kilonensis JX22 against Fusarium oxysporum f. sp. lycopersici
Author(s) -
Xu Jianhong,
Deng Peng,
Showmaker Kurt C.,
Wang Hui,
Baird Sonya M.,
Lu ShiEn
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12411
Subject(s) - fusarium oxysporum , mutant , biology , microbiology and biotechnology , transposable element , strain (injury) , gene , pseudomonas , antimicrobial , antifungal , fusarium oxysporum f.sp. lycopersici , bacteria , genetics , fusarium wilt , anatomy
Strain JX22, exhibiting a broad range of antimicrobial activities to fungal pathogens, was isolated and classified as representing P seudomonas kilonensis . In this study, the mutant JX22MT1 was obtained by the EZ‐Tn5 transposon mutation and showed no antifungal activity against F usarium oxysporum f. sp. lycopersici as compared with wild‐type strain JX22. The pqqC gene was disrupted in the mutant. Antifungal activity at the wild‐type level was restored from the mutant JX22MT1 with the introduction of the functional pqqC gene, which encodes pyrroloquinoline–quinone synthesis protein C. The results suggest that pqqC is essential for antifungal activity of P. kilonensis JX22 against F. oxysporum f. sp. lycopersici .