Open AccessMobile elements and mitochondrial genome expansion in the soil fungus and potato pathogen R hizoctonia solani AG ‐3
Author(s) -
Losada Liliana,
Pakala Suman B.,
Fedorova Natalie D.,
Joardar Vinita,
Shabalina Svetlana A.,
Hostetler Jessica,
Pakala Suchitra M.,
Zafar Nikhat,
Thomas Elizabeth,
RodriguezCarres Marianela,
Dean Ralph,
Vilgalys Rytas,
Nierman William C.,
Cubeta Marc A.
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12387
Subject(s) - biology , rhizoctonia solani , homing endonuclease , genome , gene , mitochondrial dna , genetics , intron , fungus , pathogenic fungus , rhizoctonia , botany
The soil fungus R hizoctonia solani is an economically important pathogen of agricultural and forestry crops. Here, we present the complete sequence and analysis of the mitochondrial genome of R . solani , field isolate R hs1 AP . The genome (235 849 bp) is the largest mitochondrial genome of a filamentous fungus sequenced to date and exhibits a rich accumulation of introns, novel repeat sequences, homing endonuclease genes, and hypothetical genes. Stable secondary structures exhibited by repeat sequences suggest that they comprise functional, possibly catalytic RNA elements. RNA ‐Seq expression profiling confirmed that the majority of homing endonuclease genes and hypothetical genes are transcriptionally active. Comparative analysis suggests that the mitochondrial genome of R . solani is an example of a dynamic history of expansion in filamentous fungi.