Open AccessA novel colorimetric method for the detection of E scherichia coli using cytochrome c peroxidase‐encoding bacteriophage
Author(s) -
Hoang Hoang A.,
Abe Michiharu,
Nakasaki Kiyohiko
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12382
Subject(s) - escherichia coli , chromogenic , peroxidase , bacteriophage , cytochrome , cytochrome c , bacteria , pseudomonas aeruginosa , cytochrome c peroxidase , chemistry , substrate (aquarium) , recombinant dna , biochemistry , microbiology and biotechnology , biology , gene , enzyme , chromatography , genetics , mitochondrion , ecology
A new rapid and simple method was developed for the detection of E scherichia coli by constructing a recombinant T 4 phage carrying the cytochrome c peroxidase gene derived from S accharomyces cerevisiae ( T 4ccp) using which, the colorimetric detection of E . coli K 12 was examined. The oxidation activity toward the chromogenic substrate cytochrome c was demonstrated by the cytochrome c peroxidase ( CCP ) produced from the T 4ccp genome. The color change caused by the oxidation of the substrate could be visually perceived. The possibility of interference in the detection by the coexistence of other bacteria was assessed using P seudomonas aeruginosa as a nontarget bacterium, and it was confirmed that the coexistence of P . aeruginosa caused no interference in the detection of E . coli K 12.