Open AccessInfluence of bacteriophage P 22 on the inflammatory mediator gene expression in chicken macrophage HD 11 cells infected with S almonella T yphimurium
Author(s) -
Ahn Juhee,
Biswas Debabrata
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12379
Subject(s) - bacteriophage , biology , microbiology and biotechnology , intracellular , salmonella , immune system , salmonella enterica , gene expression , gene , virology , bacteria , escherichia coli , immunology , biochemistry , genetics
This study was designed to evaluate the effects of bacteriophage on the intracellular survival and immune mediator gene expression in chicken macrophage‐like HD 11 cells. The invasive ability and intracellular survival of S almonella T yphimurium ( ST P22− ) and lysogenic S . T yphimurium ( ST P22+ ) in HD 11 cells were evaluated at 37 °C for 24 h postinfection (hpi). The expression of inflammatory mediator genes was determined in ST P22− ‐ and ST P22+ ‐infected HD 11 cells treated with and without bacteriophage P 22 at 1 and 24 hpi using quantitative RT ‐ PCR . The ability of ST P22− and ST P22+ to invade HD 11 cells was significantly decreased by bacteriophage P 22 at 1 hpi. The numbers of intracellular ST P22− and ST P22+ were significantly decreased from 2.39 to 1.62 CFU cm −2 and from 3.40 to 1.72 CFU cm −2 in HD 11 cells treated with bacteriophage P 22, respectively, at 24 hpi. The enhanced expression of inflammatory mediators was observed in ST P22− ‐ and ST P22+ ‐infected HD 11 cells treated with and without bacteriophage P 22. These results suggest that the application of bacteriophage could be an effective way to control the intracellular infection.