Open AccessAerobic condition increases carotenoid production associated with oxidative stress tolerance in E nterococcus gilvus
Author(s) -
Hagi Tatsuro,
Kobayashi Miho,
Nomura Masaru
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12341
Subject(s) - carotenoid , anaerobic exercise , food science , biology , aerobic bacteria , lactic acid , oxidative stress , biochemistry , bacteria , chemistry , physiology , genetics
Although it is known that a part of lactic acid bacteria can produce carotenoid, little is known about the regulation of carotenoid production. The objective of this study was to determine whether aerobic growth condition influences carotenoid production in carotenoid‐producing E nterococcus gilvus . E nterococcus gilvus was grown under aerobic and anaerobic conditions. Its growth was slower under aerobic than under anaerobic conditions. The decrease in p H levels and production of lactic acid were also lower under aerobic than under anaerobic conditions. In contrast, the amount of carotenoid pigments produced by E . gilvus was significantly higher under aerobic than under anaerobic conditions. Further, real‐time quantitative reverse transcription PCR revealed that the expression level of carotenoid biosynthesis genes crtN and crtM when E . gilvus was grown under aerobic conditions was 2.55–5.86‐fold higher than when it was grown under anaerobic conditions. Moreover, after exposure to 16‐ and 32‐mM H 2 O 2 , the survival rate of E . gilvus grown under aerobic conditions was 61.5‐ and 72.5‐fold higher, respectively, than when it was grown under anaerobic conditions. Aerobic growth conditions significantly induced carotenoid production and the expression of carotenoid biosynthesis genes in E . gilvus , resulting in increased oxidative stress tolerance.