Open AccessIn vitro effectiveness of the antimicrobial peptide e CATH 1 against antibiotic‐resistant bacterial pathogens of horses
Author(s) -
Schlusselhuber Margot,
Guldbech Kristen,
Sevin Corinne,
Leippe Matthias,
Petry Sandrine,
Grötzinger Joachim,
Giguère Steeve,
Cauchard Julien
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12337
Subject(s) - microbiology and biotechnology , antimicrobial , rhodococcus equi , biology , klebsiella pneumoniae , antibiotics , staphylococcus aureus , broth microdilution , salmonella enterica , antibiotic resistance , pseudomonas aeruginosa , minimum inhibitory concentration , bacteria , escherichia coli , salmonella , biochemistry , virulence , gene , genetics
The equine antimicrobial peptide e CATH 1 previously has been shown to have in vitro activity against antibiotic‐susceptible reference strains of R hodococcus equi and common respiratory bacterial pathogens of foals. Interestingly, e CATH 1 was also found to be effective in the treatment of R . equi infection induced in mice. The aim of this study was to assess the in vitro activity of e CATH 1 against equine isolates of Gram‐negative ( E scherichia coli , S almonella enterica , K lebsiella pneumoniae and P seudomonas spp.) and G ram‐positive ( R . equi , S taphylococcus aureus ) bacteria resistant to multiple classes of conventional antibiotics. A modified microdilution method was used to evaluate the minimum inhibitory concentrations ( MIC s) of the antimicrobial peptide. The study revealed that e CATH 1 was active against all equine isolates of E . coli , S . enterica , K . pneumoniae , P seudomonas spp. and R . equi tested, with MIC s of 0.5–16 μg mL −1 , but was not active against most isolates of S . aureus . In conclusion, the activity of the equine antimicrobial peptide e CATH 1 appears to not be hampered by the antibiotic resistance of clinical isolates. Thus, the data suggest that e CATH 1 could be useful, not only in the treatment of R . equi infections, but also of infections caused by multidrug‐resistant G ram‐negative pathogens.