Open AccessDiscovery of two novel β‐glucosidases from an A mazon soil metagenomic library
Author(s) -
Bergmann Jessica C.,
Costa Ohana Yonara A.,
Gladden John M.,
Singer Steven,
Heins Richard,
D'haeseleer Patrik,
Simmons Blake A.,
Quirino Betania F.
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12332
Subject(s) - fosmid , metagenomics , glucosidases , orfs , amazon rainforest , biology , enzyme , microbiology and biotechnology , biochemistry , gene , ecology , open reading frame , peptide sequence
An A mazon soil microbial community metagenomic fosmid library was functionally screened for β‐glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β‐glucosidases, AmBGL17 and AmBGL18, from the GH3 and GH1 families, respectively. Both AmBGL17 and AmBGL18 were functionally identified as β‐glucosidases. The enzymatic activity of AmBGL17 was further characterized. AmBGL17 was tested with different substrates and showed highest activity on pNPβG substrate with an optimum temperature of 45 °C and an optimum pH of 6. AmBGL17 showed a V max of 116 mM s −1 and K m of 0.30 ± 0.017 mM. This is the first report of β‐glucosidases from an Amazon soil microbial community using a metagenomic approach.