Open AccessNovel A mycolatopsis balhimycina biochemical abilities unveiled by proteomics
Author(s) -
Alduina Rosa,
Gallo Giuseppe,
Renzone Gianni,
Weber Tilmann,
Scaloni Andrea,
Puglia Anna M.
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12324
Subject(s) - proteomics , computational biology , biology , chemistry , biochemistry , gene
A mycolatopsis balhimycina DSM 5908 is an actinomycete producer of balhimycin, an analogue of vancomycin, the antibiotic of ‘last resort’ against multidrug‐resistant Gram‐positive pathogens. Most knowledge on glycopeptide biosynthetic pathways comes from studies on A . balhimycina as this strain, among glycopeptide producers, is genetically more amenable. The recent availability of its genome sequence allowed to perform differential proteomic analyses elucidating key metabolic pathways leading to antibiotic production in different growth conditions. To implement proteomic data on A . balhimycina derived from 2‐ DE approaches and to identify novel components, a combined approach based on protein extraction with different detergents, SDS ‐ PAGE resolution of intact proteins and nano LC ‐ ESI ‐ LIT ‐ MS / MS analysis of their tryptic digests was carried out. With this procedure, 206 additional new proteins such as very basic, hydrophobic or large species were identified. This analysis revealed either components whose expression was previously only inferred by growth conditions, that is, those involved in glutamate metabolism or in resistance, or proteins that allow the strain to metabolize alkanes. These findings will give additional insight into metabolic pathways that could really contribute to A . balhimycina growth and antibiotic production and metabolic enzymes that could be manipulated to generate a model producing strain to use for synthetic biology.