Open AccessExploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture
Author(s) -
Desriac Florie,
Chevalier Patrick,
Brillet Benjamin,
Leguerinel Ivan,
Thuillier Benoît,
Paillard Christine,
Fleury Yannick
Publication year - 2014
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12308
Subject(s) - hemolymph , biology , mytilus , crassostrea , microbiology and biotechnology , aquaculture , bacteria , bivalvia , shellfish , marine bacteriophage , zoology , ecology , aquatic animal , fishery , mollusca , oyster , genetics , fish <actinopterygii>
Haemolymph‐associated microbiota of marine bivalves was explored for antibacterial activity against important aquaculture pathogens. A collection of 843 strains were cultured from the haemolymph of four bivalve species ( C rassostrea gigas , M ytilus edulis , P ecten maximus and T apes rhomboides ) collected by deep‐sea diving in the G lenan A rchipelago ( F rance). Cell‐free culture supernatants were investigated for antibacterial activity using the well‐diffusion assay. About 3% of haemolymph‐associated cultivable bacteria displayed antibacterial activity toward Gram‐negative pathogens. Among the active bacteria, P seudoalteromonas strains exhibited the highest antibacterial activity. The cell‐free culture supernatant of one of them, named h C g ‐51, was able to inhibit the growth of bacterial pathogens even after drastic dilution (1 : 1024). Hemocyte survival was not significantly altered in the presence of the haemolymph‐associated strains assayed. Moreover, a dose‐dependent beneficial effect on hemocyte survival rates was observed with the h C g ‐51 strain. These results suggest that haemolymph microbiota may participate in bivalve protection and therefore confer a health benefit on the host. As a result, the results highlight bivalve haemolymph microbiota as a promising novel source for aquaculture probiotics. This work also gives a first insight into the contribution of the haemolymph‐associated microbiota as part of the bivalve ‘hologenome’.