Open AccessHydrogen‐oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH
Author(s) -
Poladyan Anna,
Trchounian Karen,
Sawers R. Gary,
Trchounian Armen
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12281
Subject(s) - hydrogenase , oxidizing agent , escherichia coli , fermentation , redox , chemistry , atpase , biochemistry , mutant , enzyme , inorganic chemistry , organic chemistry , gene
Simultaneous measurement of redox potential ( E h ) and determination of H 2 evolution kinetics using a pair of titanium silicate and platinum redox electrodes in fermenting cultures of E scherichia coli wild type and different mutants lacking hydrogenases 1 (Hyd‐1) or 2 (Hyd‐2) revealed that Hyd‐1 controls the onset of H 2 evolution at slightly alkaline pH ( pH 7.5) and under oxidizing E h . In addition, Hyd‐2 influences the N,N' ‐dicyclohexylcarbodiimide‐inhibited ATP ase activity in fermenting cells and thus regulates the proton F 0 F 1 ‐ATPase at the alkaline pH but under reducing E h .