Open AccessRaman spectrometric discrimination of flexirubin pigments from two genera of B acteroidetes
Author(s) -
Jehlička Jan,
Osterrothová Kateřina,
Oren Aharon,
Edwards Howell G. M.
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12243
Subject(s) - bacteroidetes , pigment , flavobacterium , raman spectroscopy , biology , bacteria , chemistry , 16s ribosomal rna , optics , paleontology , physics , organic chemistry , pseudomonas
Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes . Colonies and cell extracts of F lavobacterium johnsoniae and F lexibacter elegans have been investigated by R aman spectroscopy to show that this fast and non‐destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. The presence or absence of certain distinguishing features in the CH combination band region at 2500–2750 cm −1 can assist in the discrimination between the two flexirubins investigated. Raman spectroscopy is thus a suitable tool not only to detect flexirubin pigments in bacterial cells, but also to further characterize the pigments present in members of the B acteroidetes genera that are rich in flexirubins.