Open AccessCharacterization of bacterial communities associated with the pine sawyer beetle M onochamus galloprovincialis , the insect vector of the pinewood nematode B ursaphelenchus xylophilus
Author(s) -
Vicente Cláudia S.L.,
Nascimento Francisco X.,
Espada Margarida,
Barbosa Pedro,
Hasegawa Koichi,
Mota Manuel,
Oliveira Solange
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12232
Subject(s) - bursaphelenchus xylophilus , biology , xylophilus , wilt disease , pinus pinaster , longhorn beetle , proteobacteria , botany , ecology , nematode , bacteria , 16s ribosomal rna , genetics
Pine wilt disease ( PWD ) has a tremendous impact on worldwide forestlands, both from the environmental and economical viewpoints. M onochamus sp., a xylophagous insect from the C erambycidae family, plays an important role in dissemination of the pinewood nematode, B ursaphelenchus xylophilus , the primary pathogenic agent of PWD . This study investigates, for the first time, the bacterial communities of M onochamus galloprovincialis collected from Portuguese Pinus pinaster trees and B . xylophilus free, using a metagenomics approach. Overall, our results show that natural bacterial communities of M . galloprovincialis are mainly composed by γ‐proteobacteria, F irmicutes and B acteroidetes , which may be a reflection of insects' feeding diet and habitat characteristics. We also report different bacterial communities' composition in the thorax and abdomen of M . galloprovincialis , with high abundance of S erratia sp. in both. Our results encourage further studies in the possible relationship between bacteria from the insect vector and B . xylophilus .