Open AccessCharacterization of depth‐related changes in bacterial community compositions and functions of a paddy soil profile
Author(s) -
Huang Jing,
Sheng Xiafang,
He Linyan,
Huang Zhi,
Wang Qi,
Zhang Zhendong
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12218
Subject(s) - edaphic , temperature gradient gel electrophoresis , soil horizon , biogeochemical cycle , soil carbon , biology , microbial population biology , relative species abundance , abundance (ecology) , canonical correspondence analysis , community structure , soil water , soil test , agronomy , bacteria , ecology , genetics , 16s ribosomal rna
Depth‐related changes in bacterial community structures and functions were analyzed in a paddy soil profile using denaturing gradient gel electrophoresis ( DGGE ) and a metabolic profiling technique ( BIOLOG ECO plates). Canonical correspondence analysis ( CCA ) was used to analyze the correlations between the relative abundance of bacterial groups and soil‐available elements. DGGE and sequencing analysis revealed 12 classes and one unknown bacterial group. At the family level, C omamonadaceae and M oraxellaceae dominated through the soil profile, while A cidobacteriaceae and N itrospiraceae dominated in the deepest layer. In addition, S treptococcaceae dominated and was only observed in the deeper layers. Metabolic profiles revealed the greatest carbon source utilization capacity in the surface layer, and no significant differences between upper and deeper soil layers. The carbon sources utilized by microorganisms were different among the different layers. CCA indicated that soil‐available M n, C a, C u, A l, and K concentrations were positively correlated with the relative abundance of C omamonadaceae , M oraxellaceae , S treptococcaceae , M icrobacteriaceae , N ocardioidaceae , and N itrospiraceae in the profile. The results showed that the paddy soil profile harbored diverse bacterial communities and experienced depth‐related changes in community structure and carbon source utilization. The bacterial communities and functions might be shaped by the soil edaphic characteristics along the soil profile.