Open AccessPreparative‐cum‐quantitative mass‐directed analysis of swainsonine and its in situ activity against Sf ‐21 cell line
Author(s) -
Singh Digar,
Kaur Gurvinder
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12214
Subject(s) - swainsonine , indolizidine , chromatography , acetone , chemistry , quantitative analysis (chemistry) , biology , alkaloid , stereochemistry , biochemistry
Swainsonine is a polyhydroxy indolizidine alkaloid with various research and potential therapeutic applications. In this work, swainsonine was partially purified (2.5‐folds) with acetone–methanol solvent system from M etarhizium anisopliae fermentation broth. The partially purified broth was further subjected to mass‐directed preparative‐cum‐quantitative analysis. Swainsonine was eluted as MS 1 fraction [M + H] + 174.36 ± 0.21 at 4.91 ± 0.04 min with calculated yield of 7.85 ± 1.59 μg mL −1 corresponding to 3.74 × 10 5 counts. In situ antiproliferative activity of standard and purified swainsonine fractions was tested against S podoptera frugiperda, Sf ‐21 cell line with IC 50 values of 2.96 μM and 3.28 μM, respectively, at 36 h. This analytical procedure for purification and quantitative analysis of swainsonine may ensure its suitability for routine laboratory studies and research.