Open AccessAmoebicidal activity of phytosynthesized silver nanoparticles and their in vitro cytotoxicity to human cells
Author(s) -
Borase Hemant P.,
Patil Chandrashekhar D.,
Sauter Ismael P.,
Rott Marilise B.,
Patil Satish V.
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12195
Subject(s) - jatropha curcas , euphorbiaceae , cytotoxicity , silver nanoparticle , in vitro , microbiology and biotechnology , acanthamoeba , traditional medicine , viability assay , biology , antimicrobial , peripheral blood mononuclear cell , chemistry , botany , biochemistry , nanoparticle , medicine , nanotechnology , materials science
A canthamoeba causes infections in humans and other animals and it is important to develop treatment therapies. J atropha curcas , J atropha gossypifolia and E uphorbia milii plant extracts synthesized stable silver nanoparticles ( A g NP s) that were relatively stable. Amoebicidal activity of J . gossypifolia , J . curcas and E . milii leaf extracts showed little effect on viability of A canthamoeba castellanii trophozoites. Plant‐synthesized A g NP s showed higher amoebicidal activity. A g NP s synthesized by J . gossypifolia extract were able to kill 74–27% of the trophozoites at concentrations of 25–1.56 μg mL −1 . A g NP s were nontoxic at minimum inhibitory concentration with peripheral blood mononuclear cells. These results suggest biologically synthesized nanoparticles as an alternative candidate for treatment of A canthamoeba infections.