Open AccessDNA replication depends on photosynthetic electron transport in cyanobacteria
Author(s) -
Ohbayashi Ryudo,
Watanabe Satoru,
Kanesaki Yu,
Narikawa Rei,
Chibazakura Taku,
Ikeuchi Masahiko,
Yoshikawa Hirofumi
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12166
Subject(s) - dcmu , dna replication , origin recognition complex , control of chromosome duplication , replication factor c , biology , dna , dnaa , eukaryotic dna replication , photosynthesis , dna replication factor cdt1 , microbiology and biotechnology , chemistry , genetics , biochemistry , photosystem ii
The freshwater cyanobacterium S ynechococcus elongatus PCC 7942 exhibits light‐dependent growth. Although it has been reported that DNA replication also depends on light irradiation in S . elongatus 7942, the involvement of the light in the regulation of DNA replication remains unclear. To elucidate the regulatory pathway of DNA replication by light, we studied the effect of several inhibitors, including two electron transport inhibitors, 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea ( DCMU ) and 2,5‐dibromo‐3‐methyl‐6‐isopropyl‐ p ‐benzoquinone ( DBMIB ), on DNA replication in S . elongatus 7942. DCMU inhibited only DNA replication initiation, whereas DBMIB blocked both the initiation and progression of DNA replication. These results suggest that DNA replication depends on the photosynthetic electron transport activity and initiation and progression of DNA replication are regulated in different ways.