Open AccessRN ase III ‐dependent down‐regulation of ftsH by an artificial internal sense RNA in Anabaena sp. PCC 7120
Author(s) -
Gao Yuan,
Gong Yangmin,
Xu Xudong
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12165
Subject(s) - degradosome , rnase p , rna , rnase h , biology , sense (electronics) , rna silencing , non coding rna , antisense rna , biochemistry , mutant , rna interference , microbiology and biotechnology , gene , chemistry
RN ase III is a group of ds RNA ‐specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC 7120 are highly similar to RN ase III enzymes. In vitro , recombinant Alr0280 showed RN ase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA ( aaftsH ) that was complementary to aftsH , an internal antisense RNA . The aaftsH interference was abolished by inactivation of alr0280 , the RN ase III ‐encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1 , an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RN ase III ‐dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.