Open AccessGenome sequences of two dehalogenation specialists – D ehalococcoides mccartyi strains BTF 08 and DCMB 5 enriched from the highly polluted B itterfeld region
Author(s) -
Pöritz Marlén,
Goris Tobias,
Wubet Tesfaye,
Tarkka Mika T.,
Buscot François,
Nijenhuis Ivonne,
Lechner Ute,
Adrian Lorenz
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12160
Subject(s) - dehalogenase , genome , gene , dehalococcoides , biology , reductive dechlorination , genetics , strain (injury) , vinyl chloride , chemistry , contamination , ecology , organic chemistry , anatomy , copolymer , polymer
The genomes of two novel D ehalococcoides mccartyi strains, DCMB 5 and BTF 08, enriched from the heavily organohalide‐contaminated megasite around B itterfeld ( G ermany), were fully sequenced and annotated. Although overall similar, the genome sequences of the two strains reveal remarkable differences in their genetic content, reflecting a specific adaptation to the contaminants at the field sites from which they were enriched. The genome of strain BTF 08 encodes for 20 reductive dehalogenases, and is the first example of a genome containing all three enzymes that are necessary to couple the complete reductive dechlorination of PCE to ethene to growth. The genes encoding trichloroethene and vinyl chloride reductive dehalogenases, tceA and vcrA , are located within mobile genetic elements, suggesting their recent horizontal acquisition. The genome of strain DCMB 5 contains 23 reductive dehalogenase genes, including cbrA , which encodes a chlorobenzene reductive dehalogenase, and a gene cluster encoding arsenic resistance proteins, both corresponding to typical pollutants at its isolation site.