Open AccessEvaluation and implementation of a membrane filter method for Cronobacter detection in drinking water
Author(s) -
Liu Hui,
Yang Yuelian,
Cui Jinghua,
Liu Lanzheng,
Liu Huiyuan,
Hu Guangchun,
Shi Yuwen,
Li Jian
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12155
Subject(s) - cronobacter , cronobacter sakazakii , membrane filter , contamination , microbiology and biotechnology , chemistry , chromatography , isolation (microbiology) , environmental science , food science , environmental chemistry , biology , infant formula , membrane , enterobacter , escherichia coli , ecology , biochemistry , gene
A membrane filter ( MF ) method was evaluated for its suitability for qualitative and quantitative analyses of C ronobacter spp. in drinking water by pure strains of C ronobacter and non‐ C ronobacter , and samples spiked with chlorinated C ronobacter sakazakii ATCC 29544. The applicability was verified by the tests: for pure strains, the sensitivity and the specificity were both 100%; for spiked samples, the MF method recovered 82.8 ± 10.4% chlorinated ATCC 29544 cells. The MF method was also applied to screen C ronobacter spp. in drinking water samples from municipal water supplies on premises ( MWSP ) and small community water supplies on premises ( SCWSP ). The isolation rate of C ronobacter spp. from SCWSP samples was 31/114, which was significantly higher than that from MWSP samples which was 1/131. Besides, the study confirmed the possibility of using total coliform as an indicator of contamination level of C ronobacter spp. in drinking water, and the acquired correct positive rate was 96%.