Open AccessK lebsiella sp. strain C 2 A isolated from olive oil mill waste is able to tolerate and degrade tannic acid in very high concentrations
Author(s) -
Pepi Milva,
Cappelli Serena,
Hachicho Nancy,
Perra Guido,
Renzi Monia,
Tarabelli Alessandro,
Altieri Roberto,
Esposito Alessandro,
Focardi Silvano E.,
Heipieper Hermann J.
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12136
Subject(s) - tannic acid , gallic acid , tannase , food science , chemistry , klebsiella , strain (injury) , oleic acid , bacteria , 16s ribosomal rna , fatty acid , polyphenol , microbiology and biotechnology , biochemistry , biology , organic chemistry , escherichia coli , antioxidant , genetics , anatomy , gene
Abstract Four bacterial strains capable of growing in the presence of tannic acid as sole carbon and energy source were isolated from olive mill waste mixtures. 16 S r RNA gene sequencing assigned them to the genus K lebsiella . The most efficient strain, K lebsiella sp. strain C 2 A , was able to degrade 3.5 g L −1 tannic acid within 35 h with synthesizing gallic acid as main product. The capability of K lebsiella sp. strain C 2 A to produce tannase was evidenced at high concentrations of tannic acid up to 50 g L −1 . The bacteria adapted to the toxicity of tannic acids by an increase in the membrane lipid fatty acids degree of saturation, especially in the presence of concentrations higher than 20 g L −1 . The highly tolerant and adaptable bacterial strain characterized in this study could be used in bioremediation processes of wastes rich in polyphenols such as those derived from olive mills, winery or tanneries.