Open AccessAn overview of the metabolic differences between B radyrhizobium japonicum 110 bacteria and differentiated bacteroids from soybean ( Glycine max ) root nodules: an in vitro 13 C‐ and 31 P‐nuclear magnetic resonance spectroscopy study
Author(s) -
Vauclare Pierre,
Bligny Richard,
Gout Elisabeth,
Widmer François
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12124
Subject(s) - bradyrhizobium japonicum , rhizobia , bradyrhizobium , root nodule , glycine , bacteria , metabolite , nitrogen fixation , biochemistry , symbiosis , biology , trehalose , legume , rhizobiaceae , inositol , botany , amino acid , genetics , receptor
B radyrhizobium japonicum is a symbiotic nitrogen‐fixing soil bacteria that induce root nodules formation in legume soybean ( G lycine max .). Using 13 C‐ and 31 P‐nuclear magnetic resonance ( NMR ) spectroscopy, we have analysed the metabolite profiles of cultivated B . japonicum cells and bacteroids isolated from soybean nodules. Our results revealed some quantitative and qualitative differences between the metabolite profiles of bacteroids and their vegetative state. This includes in bacteroids a huge accumulation of soluble carbohydrates such as trehalose, glutamate, myo‐inositol and homospermidine as well as Pi, nucleotide pools and intermediates of the primary carbon metabolism. Using this novel approach, these data show that most of the compounds detected in bacteroids reflect the metabolic adaptation of rhizobia to the surrounding microenvironment with its host plant cells.