Open AccessA transposon‐based strategy to identify the regulatory gene network responsible for landomycin E biosynthesis
Author(s) -
Horbal Lilya,
Fedorenko Viktor,
Bechthold Andreas,
Luzhetskyy Andriy
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12117
Subject(s) - actinorhodin , streptomyces coelicolor , subcloning , transposon mutagenesis , biology , transposable element , tetr , mutant , genetics , gene , streptomyces , promoter , regulator gene , mutagenesis , regulation of gene expression , plasmid , transcription factor , gene expression , repressor , bacteria
We report here a transposon‐based strategy to generate S treptomyces globisporus 1912 mutants with improved landomycin E production. The modified minitransposon with strong, outward‐oriented promoters for the overexpression of downstream‐situated genes has been applied for mutant library generation. Approximately 2500 mutants of S . globisporus 1912 were analyzed for landomycin E production, leading to the identification of several overproducers. Subcloning and sequencing of the sites of integration showed that some of the inactivated genes encode proteins with a similarity to known bacterial regulators such as TetR and LuxR families. One of the regulators (GntR type) has shown the strongest influence on the landomycin E production. Its ortholog (encoded by sco3269 ) in S treptomyces coelicolor was characterized in greater detail and showed similar effects on actinorhodin production and morphological differentiation.