Open AccessA novel manganese starvation‐inducible expression system for Lactobacillus plantarum
Author(s) -
Böhmer Nico,
König Saskia,
Fischer Lutz
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12105
Subject(s) - pyrococcus furiosus , lactobacillus plantarum , expression vector , manganese , biology , chemistry , gene , microbiology and biotechnology , biochemistry , bacteria , lactic acid , genetics , recombinant dna , organic chemistry , archaea
A novel expression system for L actobacillus plantarum was developed. This system is based on the manganese starvation‐inducible promoter from specific manganese transporter of L . plantarum NC 8, which was cloned for the first time. The expression of a β ‐glucosidase from P yrococcus furiosus (CelB) was achieved by cultivating L . plantarum NC 8 at low manganese concentrations with MRS medium and the pmntH2‐CelB expression vector. A CelB activity of 8.52 μkat o NPGal L −1 was produced in a bioreactor (4 L). The advantages of the novel expression system are that no addition of an external inducing agent was required, and additionally, no further introduction of regulatory genes was necessary. The new promoter meets the general demands of a food‐grade expression system.