Open AccessTwo ABC transporter systems participate in siderophore transport in the marine pathogen V ibrio anguillarum 775 ( pJM 1)
Author(s) -
Naka Hiroaki,
Liu Moqing,
Crosa Jorge H.
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12092
Subject(s) - vibrio anguillarum , siderophore , enterobactin , ferric , atp binding cassette transporter , biology , mutant , gene , transporter , plasmid , vibrio cholerae , genetics , microbiology and biotechnology , vibrio , biochemistry , chemistry , bacteria , organic chemistry
ORF40 (named fatE ) in the Vibrio anguillarum pJM 1 plasmid‐encoding anguibactin iron transport systems is a homolog of ATPase genes involved in ferric‐siderophore transport. Mutation of fatE did not affect ferric‐anguibactin transport, indicating that there must be other ATPase gene(s) in addition to fatE . By searching the genomic sequence of V. anguillarum 775( pJM 1), we identified a homolog of fatE named fvtE on chromosome 2. It is of interest that in this locus, we also identified homologs of fatB , fatC, and fatD that we named fvtB , fvtC and fvtD , respectively. The fvtE mutant still showed ferric‐anguibactin transport, while the double fatE and fvtE mutation completely abolished the ferric‐anguibactin transport indicating that fatE and fvtE are functional ATPase homologs for ferric‐anguibactin transport. Furthermore, we demonstrate that fvtB , fvtC , fvtD, and fvtE are essential for ferric‐vanchrobactin and ferric‐enterobactin transport.