Open AccessA mp G is required for B la Xc beta‐lactamase expression in X anthomonas campestris pv. campestris str. 17
Author(s) -
Yang TsueyChing,
Chen TzuFan,
Tsai Jeffrey J.P.,
Hu RouhMei
Publication year - 2013
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12071
Subject(s) - xanthomonas campestris , biology , microbiology and biotechnology , mutant , escherichia coli , inducer , stenotrophomonas maltophilia , xanthomonas campestris pv. campestris , bacteria , gene , pseudomonas aeruginosa , genetics
The chromosomal amp R Xc ‐ bla Xc module is essential for the β‐lactam resistance of X anthomonas campestris pv. campestris. B la Xc β‐lactamase is expressed at a high basal level in the absence of an inducer and its expression can be further induced by β‐lactam. In enterobacteria, amp G encodes an inner membrane facilitator involved in the recycling of murein degradation compounds. An isogenic amp G mutant ( X camp G ) of X . campestris pv. campestris str. 17 ( X c17) was constructed to investigate the link between murein recycling and bla Xc expression. Our data demonstrate that (1) X camp G is susceptible to β‐lactam antibiotics; (2) A mp G Xc is essential for expression of bla Xc ; (3) A mp G s of X c17, S tenotrophomonas maltophilia KJ ( S m KJ ) and E scherichia coli DH 5α can complement the defect of X camp G ; (4) overexpression of A mp G X c significantly increased bla Xc expression; and (5) A mp G Xc from X c17 is able to restore β‐lactamase induction of the amp N Xc ‐ amp G Xc double mutant of S m KJ . In X c17, amp G Xc can be expressed from the promoter residing in the intergenic region of amp N Xc ‐ amp G Xc and the expression is independent of β‐lactam induction. A mp N , which is required for β‐lactamases induction in S m KJ , is not required for the β‐lactam antibiotic resistance of X c17.