Open AccessCopper induction of enhanced green fluorescent protein expression in P leurotus ostreatus driven by laccase poxa1b promoter
Author(s) -
Amore Antonella,
Honda Yoichi,
Faraco Vincenza
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12023
Subject(s) - laccase , pleurotus ostreatus , promoter , transformation (genetics) , green fluorescent protein , chemistry , biochemistry , fluorescence , microbiology and biotechnology , gene expression , biology , gene , enzyme , food science , mushroom , physics , quantum mechanics
Abstract In silico analyses of several laccase promoter sequences have shown the presence of many different responsive elements differentially distributed along the promoter sequences. Analysis of P leurotus ostreatus laccase promoter poxa1b extending around 1400‐bp upstream of the start codon showed the presence of several putative response elements, such as 10 metal‐responsive elements. Development of a system for in vivo analysis of P . ostreatus laccase promoter poxa1b by enhanced green fluorescent protein expression was carried out, based on a polyethylene glycol–mediated procedure for fungal transformation. Quantitative measurement of fluorescence expressed in P . ostreatus transformants grown in the presence and in the absence of copper sulfate was performed, demonstrating an increase in expression level induced by the metal.