Open AccessComparison of dkgB ‐linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to S almonella enterica
Author(s) -
Guard Jean,
SanchezIngunza Roxana,
Morales Cesar,
Stewart Tod,
Liljebjelke Karen,
Kessel JoAnn,
Ingram Kim,
Jones Deana,
Jackson Charlene,
FedorkaCray Paula,
Frye Jonathan,
Gast Richard,
Hinton Arthur
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12010
Subject(s) - ribotyping , serotype , biology , salmonella enterica , intergenic region , dna microarray , gene , genetics , salmonella , typing , microbiology and biotechnology , polymerase chain reaction , virology , genome , escherichia coli , gene expression , bacteria
Two DNA‐based methods were compared for the ability to assign serotype to 139 isolates of S almonella enterica ssp. I . Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB . A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than K auffmann– W hite serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S . enterica while maintaining reliability may encourage routine testing and research.