Open AccessA novel gene, ardD , determines antirestriction activity of the non‐conjugative transposon Tn 5053 and is located antisense within the tniA gene
Author(s) -
Balabanov Vladimir P.,
Kotova Vera Yu.,
Kholodii Gennady Y.,
Mindlin Sofia Z.,
Zavilgelsky Gennadii B.
Publication year - 2012
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/1574-6968.12005
Subject(s) - transposable element , transposase , gene , biology , genetics , transcription (linguistics) , coding region , restriction enzyme , microbiology and biotechnology , sleeping beauty transposon system , mutant , linguistics , philosophy
The mercury‐resistance transposon Tn 5053 inhibits restriction activity of the type I restriction‐modification endonuclease E co KI in E scherichia coli K 12 cells. This is the first report of antirestriction activity of a non‐conjugative transposon. The gene ( ardD ) coding for the antirestriction protein has been cloned. The ardD gene is located within the tniA gene, coding for transposase, on the complementary strand. The direction of transcription is opposite to transcription of the tniA gene.