Open AccessMannose receptor, C type 1 contributes to bacterial uptake by placental trophoblast giant cells
Author(s) -
Hashino Masanori,
Tachibana Masato,
Shimizu Takashi,
Watarai Masahisa
Publication year - 2012
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/1574-695x.12009
Subject(s) - biology , mannose receptor , trophoblast , bacteria , microbiology and biotechnology , immune system , macrophage , giant cell , receptor , phagocytosis , placenta , biochemistry , in vitro , immunology , fetus , pregnancy , genetics
During pregnancy, maternal immune function is strictly controlled and immune tolerance is induced. Trophoblast giant ( TG ) cells exhibit phagocytic activity and show macrophage‐like activity against microorganisms in the placenta. However, details of molecular receptors and mechanisms for uptake by TG cells have not been clarified. In this study, we investigated the involvement of the mannose receptor, C type 1 ( MRC 1), in the uptake of the abortion‐inducible bacterium L isteria monocytogenes and abortion‐uninducible bacteria B acillus subtilis and E scherichia coli by TG cells differentiated from a mouse trophoblast stem cell line in vitro . Knockdown of MRC 1 inhibited the uptake of all of these bacteria, as did the blocking of MRC 1 by MRC 1 ligands. The uptake of bacteria by MRC 1 delayed the maturation of phagolysosomes. These findings suggest that MRC 1 plays an important role in the uptake of various bacteria by TG cells and may provide an opportunity for those bacteria to escape from phagosomes.