Open AccessSoil type‐dependent effects of a potential biocontrol inoculant on indigenous bacterial communities in the rhizosphere of field‐grown lettuce
Author(s) -
Schreiter Susanne,
Ding GuoChun,
Grosch Rita,
Kropf Siegfried,
Antweiler Kai,
Smalla Kornelia
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12430
Subject(s) - rhizosphere , biology , microbial inoculant , loam , pyrosequencing , temperature gradient gel electrophoresis , soil type , agronomy , botany , 16s ribosomal rna , soil water , horticulture , ecology , inoculation , bacteria , biochemistry , genetics , gene
Bacterial biocontrol strains used as an alternative to chemical fungicides may influence bacterial communities in the rhizosphere and effects might differ depending on the soil type. Here we present baseline data on the effects of Pseudomonas jessenii RU47 on the bacterial community composition in the rhizosphere of lettuce grown in diluvial sand, alluvial loam and loess loam at the same field site. 16S r RNA gene fragments amplified from total community DNA were analyzed by denaturing gradient gel electrophoresis ( DGGE ) and pyrosequencing. DGGE fingerprints revealed that in three consecutive years (2010–2012) RU 47 had a slight but statistically significant effect on the bacterial community composition in one (2010), two (2011) or all the three soils (2012). However, these effects were much less pronounced compared with the influence of soil types. Additional pyrosequence analysis of samples from 2011 showed that significant changes in bacterial community compositions in response to RU 47 inoculation occurred only in alluvial loam . Different taxonomic groups responded to the RU 47 application depending on the soil type. Most remarkable was the increased relative abundance of OTU s belonging to the genera B acillus and P aenibacillus in alluvial loam . Pyrosequencing allowed side‐effects of the application of bacterial inoculants into the rhizosphere to be identified.