Open AccessAbundance and community structure of ammonia‐oxidizing Archaea and Bacteria in response to fertilization and mowing in a temperate steppe in Inner Mongolia
Author(s) -
Chen YongLiang,
Hu HangWei,
Han HongYan,
Du Yue,
Wan ShiQiang,
Xu ZhuWen,
Chen BaoDong
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12336
Subject(s) - biology , archaea , abundance (ecology) , community structure , ecology , human fertilization , relative species abundance , temperate climate , library , terminal restriction fragment length polymorphism , botany , microbial population biology , agronomy , restriction fragment length polymorphism , bacteria , 16s ribosomal rna , genetics , polymerase chain reaction , gene
Based on a 6‐year field trial in a temperate steppe in Inner Mongolia, we investigated the effects of nitrogen (N) and phosphorus (P) fertilization and mowing on the abundance and community compositions of ammonia‐oxidizing Bacteria ( AOB ) and Archaea ( AOA ) upon early (May) and peak (August) plant growth using quantitative PCR (q PCR ), terminal‐restriction fragment length polymorphism ( T ‐ RFLP ), cloning and sequencing. The results showed that N fertilization changed AOB community composition and increased AOB abundance in both May and August, but significantly decreased AOA abundance in May. By contrast, P fertilization significantly influenced AOB abundance only in August. Mowing significantly decreased AOA abundance and had little effect on AOA community compositions in May, while significantly influencing AOB abundance in both May and August, Moreover, AOA and AOB community structures showed obvious seasonal variations between May and August. Phylogenetic analysis showed that all AOA sequences fell into the Nitrososphaera cluster, and the AOB community was dominated by Nitrosospira Cluster 3. The results suggest that fertilization and mowing play important roles in affecting the abundance and community compositions of AOA and AOB .