Open AccessPlant age and genotype affect the bacterial community composition in the tuber rhizosphere of field‐grown sweet potato plants
Author(s) -
Marques Joana M.,
Silva Thais F.,
Vollu Renata E.,
Blank Arie F.,
Ding GuoChun,
Seldin Lucy,
Smalla Kornelia
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12313
Subject(s) - rhizosphere , biology , pyrosequencing , temperature gradient gel electrophoresis , pseudomonas , botany , genotype , stenotrophomonas , 16s ribosomal rna , bacteria , horticulture , gene , genetics
The hypothesis that sweet potato genotypes containing different starch yields in their tuberous roots can affect the bacterial communities present in the rhizosphere (soil adhering to tubers) was tested in this study. Tuberous roots of field‐grown sweet potato of genotypes IPB ‐149 (commercial genotype), IPB ‐052, and IPB ‐137 were sampled three and six months after planting and analyzed by denaturing gradient gel electrophoresis ( DGGE ) and pyrosequencing analysis of 16 S r RNA genes PCR ‐amplified from total community DNA . The statistical analysis of the DGGE fingerprints showed that both plant age and genotypes influenced the bacterial community structure in the tuber rhizosphere. Pyrosequencing analysis showed that the IPB ‐149 and IPB ‐052 (both with high starch content) displayed similar bacterial composition in the tuber rhizosphere, while IPB ‐137 with the lowest starch content was distinct. In comparison with bulk soil, higher 16 S r RNA gene copy numbers (q PCR ) and numerous genera with significantly increased abundance in the tuber rhizosphere of IPB ‐137 ( S phingobium, P seudomonas, A cinetobacter, S tenotrophomonas, C hryseobacterium ) indicated a stronger rhizosphere effect. The genus B acillus was strongly enriched in the tuber rhizosphere samples of all sweet potato genotypes studied, while other genera showed a plant genotype‐dependent abundance. This is the first report on the molecular identification of bacteria being associated with the tuber rhizosphere of different sweet potato genotypes.