Open Access13 C pulse‐chase labeling comparative assessment of the active methanogenic archaeal community composition in the transgenic and nontransgenic parental rice rhizospheres
Author(s) -
Zhu Weijing,
Lu Haohao,
Hill Jaclyn,
Guo Xiaohui,
Wang Hailong,
Wu Weixiang
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12261
Subject(s) - biology , genetically modified rice , botany , poaceae , composition (language) , transgene , genetically modified crops , gene , biochemistry , linguistics , philosophy
More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a 13 C ‐ CO 2 pulse‐chase labeling experiment comparing the potential effects of cry1Ab gene transformation on 13 C tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety ( C k) and a distant parental rice variety ( D p). Results showed that 13 C partitioning in aboveground biomass (mainly in stems) and roots of D p was significantly lower than that of C k. However, there were no significant differences in 13 C partitioning between the Bt transgenic rice line ( B t) and C k. RNA ‐stable isotope probing combined with clone library analyses inferred that the group M ethanosaetaceae was the predominant methanogenic A rchaea in all three rice rhizospheres. The active methanogenic archaeal community in the B t rhizosphere was dominated by M ethanosarcinaceae , M ethanosaetaceae , and M ethanomicrobiaceae , while there were only two main methanogenic clusters ( M ethanosaetaceae and M ethanomicrobiaceae ) in the C k and D p rhizospheres. These results indicate that the insertion of cry1Ab gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere.