Open AccessBacterial community variations in an alfalfa‐rice rotation system revealed by 16 S r RNA gene 454‐pyrosequencing
Author(s) -
Lopes Ana R.,
Manaia Célia M.,
Nunes Olga C.
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12253
Subject(s) - acidobacteria , biology , pyrosequencing , actinobacteria , proteobacteria , nitrospira , bacteroidetes , bacterial phyla , chloroflexi (class) , phylum , abundance (ecology) , microbial population biology , agronomy , 16s ribosomal rna , ecology , bacteria , gene , genetics
Crop rotation is a practice harmonized with the sustainable rice production. Nevertheless, the implications of this empirical practice are not well characterized, mainly in relation to the bacterial community composition and structure. In this study, the bacterial communities of two adjacent paddy fields in the 3rd and 4th year of the crop rotation cycle and of a nonseeded subplot were characterized before rice seeding and after harvesting, using 454‐pyrosequencing of the 16 S r RNA gene. Although the phyla A cidobacteria , P roteobacteria , C hloroflexi , A ctinobacteria and B acteroidetes predominated in all the samples, there were variations in relative abundance of these groups. Samples from the 3rd and 4th years of the crop rotation differed on the higher abundance of groups of presumable aerobic bacteria and of presumable anaerobic and acidobacterial groups, respectively. Members of the phylum N itrospira were more abundant after rice harvest than in the previously sampled period. Rice cropping was positively correlated with the abundance of members of the orders A cidobacteriales and ‘ S olibacterales ’ and negatively with lineages such as C hloroflexi ‘ E llin6529’. Studies like this contribute to understand variations occurring in the microbial communities in soils under sustainable rice production, based on real‐world data.