Open AccessGenome wide profiling of A zospirillum lipoferum 4 B gene expression during interaction with rice roots
Author(s) -
Drogue Benoît,
Sanguin Hervé,
Borland Stéphanie,
PrigentCombaret Claire,
WisniewskiDyé Florence
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12244
Subject(s) - biology , microbial inoculant , gene , oryza sativa , genome , genetics , gene expression , host adaptation , gene expression profiling , botany , bacteria
A zospirillum ‐plant cooperation has been mainly studied from an agronomic point of view leading to a wide description of mechanisms implicated in plant growth‐promoting effects. However, little is known about genetic determinants implicated in bacterial adaptation to the host plant during the transition from free‐living to root‐associated lifestyles. This study aims at characterizing global gene expression of A zospirillum lipoferum 4 B following a 7‐day‐old interaction with two cultivars of O ryza sativa L . japonica (cv. C igalon from which it was originally isolated, and cv. N ipponbare). The analysis was done on a whole genome expression array with RNA samples obtained from planktonic cells, sessile cells, and root‐adhering cells. Root‐associated A zospirillum cells grow in an active sessile‐like state and gene expression is tightly adjusted to the host plant. Adaptation to rice seems to involve genes related to reactive oxygen species ( ROS ) detoxification and multidrug efflux, as well as complex regulatory networks. As revealed by the induction of genes encoding transposases, interaction with root may drive bacterial genome rearrangements. Several genes related to ABC transporters and ROS detoxification display cultivar‐specific expression profiles, suggesting host specific adaptation and raising the question of A . lipoferum 4 B /rice cv. C igalon co‐adaptation.