Open AccessOverfeeding and genetics affect the composition of intestinal microbiota in A nas platyrhynchos ( P ekin) and C airina moschata ( M uscovy) ducks
Author(s) -
Vasaï Florian,
Brugirard Ricaud Karine,
Bernadet Marie Dominique,
Cauquil Laurent,
Bouchez Olivier,
Combes Sylvie,
Davail Stéphane
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12217
Subject(s) - biology , clostridia , lachnospiraceae , bacteroidetes , ileum , firmicutes , bacteroidaceae , clostridiales , genotype , clostridiaceae , lactobacillaceae , bacteroides , lactobacillus , endocrinology , genetics , 16s ribosomal rna , bacteria , gene
To investigate the effect of overfeeding on the ileal and cecal microbiota of two genotypes of ducks ( P ekin and M uscovy), high‐throughput 16 S r RNA gene‐based pyrosequencing was used. The ducks were overfed for 12 days with 58% maize flour and 42% maize grain. Samples were collected before the overfeeding period (at 12 weeks), at 13 weeks, at 14 weeks, and 3 h after feeding. In parallel, ducks fed ad libitum were killed at the same ages. Whatever the digestive segment, the genotype, and the level of intake, F irmicutes and B acteroidetes are the dominant phyla in the bacterial community of ducks (at least 80%). Before overfeeding, ileal samples were dominated by B acilli , C lostridia , and B acteroidia classes (≥ 70%), and cecal samples, by B acteroidia and C lostridia classes (around 90%) in both P ekin and M uscovy ducks. The richness and diversity decreased in the ileum and increased in the ceca after overfeeding. Overfeeding triggers major changes in the ileum, whereas the ceca are less affected. Overfeeding increased the relative abundance of C lostridiaceae , L actobacillaceae , S treptococcaceae , and E nterococcaceae families in the ileum, whereas genotype affects particularly three families: L achnospiraceae , B acteroidaceae , and D esulfovibrionaceae in the ceca.