Open AccessQuantitative analyses of ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubis CO ) large‐subunit genes ( cbbL ) in typical paddy soils
Author(s) -
Xiao KeQing,
Bao Peng,
Bao QiongLi,
Jia Yan,
Huang FuYi,
Su JianQiang,
Zhu YongGuan
Publication year - 2014
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/1574-6941.12193
Subject(s) - rubisco , biology , soil water , ribulose , oxygenase , carbon fixation , botany , gene , photosynthesis , biochemistry , ecology
The C alvin cycle is known to be the major pathway for CO 2 fixation, but our current understanding of its occurrence and importance in paddy soils is poor. In this study, the diversity of three ribulose‐1,5‐bisphosphate carboxylase/oxygenase large‐subunit genes ( cbbL G, cbbL R, cbbM ) was investigated by clone library, T ‐ RFLP , qPCR , and enzyme assay in five paddy soils in C hina. The cbbL G sequences revealed a relatively low level of diversity and were mostly related to the sequences of species from T hiobacillus . In contrast, highly diverse cbbL R and cbbM sequences were dispersed on the phylogenetic trees, and most of them were distantly related to known sequences, even forming separate clusters. Abundances of three cbbL genes ranged from 10 6 to 10 9 copies g −1 soil, and cbbL R outnumbered cbbM and cbbL G in all soil samples, indicating that cbbL R may play a more important role than other two cbbL genes. Soil properties significantly influenced cbbL diversity in five paddy soils, of which clay content, C/N ratio, CEC, pH , and SOC correlated well with variations in microbial composition and abundance. In summary, this study provided a comparison of three cbbL genes, advancing our understanding of their role in carbon sequestration and nutrient turnover in the paddy soil.